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KMID : 0545120000100060844
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 6 p.844 ~ p.850
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Receptor-Mediated Endocytosis of Hepatitis B Virus PreS1 Protein in EBV-Transformed B-Cell Line
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Park, Jung Hyun
Cho, Eun Wie/Lee, Dong Gun/Park, Jung Min/Lee, Yun Jung/Choi, Eun A/Kim, Kil Lyong
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Abstract
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The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggesting that cellular receptors for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was localized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS 1 ligands, thereby indicating an energy-dependent receptormediated endocytotic pathway. Accordingly, the interaction of HBV-preS1 with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.
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